Preparation and Characterization of Nanobiocatalyst Microreactors with Monolithic Polymers Nanopori Enzyme Immobilized

Preparation and Characterization of Nanobiocatalyst Microreactors with Monolithic Polymers Nanopori Enzyme Immobilized

Baiq Octaviana Dwi Anggraeny, Septi F. Raeni, Elvina D. Iftitah, Akhmad Sabarudin

In this research has been done the development of nanobiokatalis mikroreaktor colonized column by trypsin enzyme in nanopore monolithic polymer. The nanopore monolithic polymer is prepared in in situ copolymerization in a silicosteel column (1.0 mm ps x 100 mm) of GMA and EDMA monomers as a crosslinker and addition of a porogen with 1-propanol / 1,4-butanediol / water composition (7: 4: 1) and AIBN’s 1% radical initiator. The polymerization process lasted for 12 hours at 60 ° C with percentage of total monomer% T 40 and percentage of crosslinker% C 25. Monolith was chemically modified using enzyme immobilization method by reaction of opening epoxide ring from monolith polymer to trypsin. Several test parameters were performed to produce the microreactor under optimum conditions ie the variation of immobilization time and the effect of addition of glutaraldehyde on the column of trypsin monolith. Characterization performed included ISEC test to find out the size and distribution of monolith pores, SEM-EDX to know morphological structure of microreactor column, and permeability test of microreactor column. Based on the results of ISEC proportion of mesoporous and macropore (flow-through pore) balanced that is 65,85% and 28,20%. The SEM-EDX results showed the highest nitrogen content representing the number of immobilized trypsin in monoliths of 12.40% produced from trypsin monolith with 4 hours immobilization time without addition of glutaraldehyde. Nanobiocatalyst microreactor under optimum conditions can be used to process digestion of protein samples quickly and efficiently.